Figure 4From: GoIFISH: a system for the quantification of single cell heterogeneity from IFISH imagesCorrelating copy number with spot area at a single cell level. Correlation between manually counted spots and automated detection of spot area using Columbus, CellProfiler and GoIFISHGoIFISH. A linear relationship between copy number and area is observed until 21 spots per cell, after which individual spots are no longer discernable by eye. Darker regions indicate a higher population of cells with similar properties.Back to article page