Figure 2
From: GoIFISH: a system for the quantification of single cell heterogeneity from IFISH images
Performance benchmarking against Columbus and CellProfiler. (A) Example of nuclear, membrane and spot segmentation using CellProfiler, Columbus, and GoIFISHGoIFISH manually corrected segmentations. (B) Average F-Scores for nuclear segmentation, membrane detection, spot detection (including clusters) in 10 sample images. (C) Differences in perimeter-area ratio of segmented nuclei and membranes compared to the gold standard. Values closer to 1 indicate similar morphology to the gold standard.