Figure 2

Methylation levels for DMRs 1–3 significantly distinguish normal kidney from Wilms tumour and whole blood. (a) Aggregated methylation levels across DMRs 1–3 significantly separate normal kidney (blue) and Wilms tumour (pink) in the test set (n = 22 pairs, P = 3.85x10⁻¹⁷) and in (b) the replication set (n = 12 pairs, P = 1.47x10⁻⁹). (c) Methylation levels were high in an independent dataset of WT including high risk (dark green, n = 25) and intermediate risk histological subtypes (light green, n = 61). High risk WT showed significantly higher methylation levels than intermediate risk WT (P = 0.0024). (d) Whole blood (n = 411) shows low methylation levels (red).