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Figure 1 | Genome Biology

Figure 1

From: MethylPurify: tumor purity deconvolution and differential methylation detection from single tumor DNA methylomes

Figure 1

Overview of MethylPurify. (a) A differentially methylated region (DMR) between tumor and normal cells. Solid and hollow red circles represent methylated and unmethylated cytosines, respectively. (b) Short reads from two cell populations after bisulfite treatment and sonication. (c) A library of bisulfite reads in a mixture of two cell populations. (d) EM algorithm iteratively estimates three parameters: the minor composition (α1) and the methylation level of each population (m1, m2) in M step, and assigns reads to each population in E step. (e) Among all 300 bp bins, the parameters estimated from informative bins converge on a final mixing ratio estimate. (f) Top, density plot of predicted minor component from selected informative bins. Bottom, separated methylation level of tumor and normal cells based on the predicted mixing ratio, and DMRs are detected as consecutive differentially methylated bins (DMBs).

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