Figure 4

Apobec-1 editing targets in relation to RNA and protein expression. (A) Schematic representation of Apobec-1-dependent editing targets in relation to RNA and protein expression. Total proteins were extracted from WT Apobec-1^-/- intestine and submitted for proteomic analysis (Materials and methods). The relative expression and editing status of the RNAs encoding the differentially expressed proteins were analyzed in parallel. Data comparison between WT and Apobec-1^-/- data sets revealed 238 Apobec-1 RNA editing targets (blue circle), 335 differentially expressed RNAs (green circle) and 893 differentially expressed proteins (orange circle). Overlapping these three groups led to the identification of only 11 edited RNAs showing altered expression concomitant with altered protein level: 10 RNAs and proteins were up-regulated in WT (blue upward arrow) and one RNA and its protein product were down-regulated in WT compared to Apobec-1^-/- (red downward arrow). (B) Reduced expression of Cd36 in intestinal extracts from Apobec-1^-/- mice. Total cell lysates from three individual WT mice and four individual Apobec-1^-/- animals were separated by SDS-PAGE probed with an anti-Cd36 and anti-α-actin antibody. * Indicates p < 0.05 for difference in protein abundance (C) Trend to increased expression of Ido1 protein expression in western blots of intestinal extracts from two individual Apobec-1^-/- mice and two individual WT mice, normalized to α-actin as a loading control. Error bars represent mean ± se of relative protein abundance by genotype.