Figure 2

RT-PCR validation of putative lncRNAs in root, leaf and shoot of 2-week seedlings of maize inbreds (B73 and Mo17). Twenty-four putative lncRNAs, including 19 HC-lncRNAs and 5 pre-lncRNAs, that exhibit expression in seedling tissue were selected for RT-PCR validation. Each primer set was used to perform RT-PCR on four RNA samples, including (1) B73 root, (2) B73 leaf, (3) B73 shoot, and (4) Mo17 shoot isolated from 2-week-old seedlings. Actin was used as a control to show amplification of cDNA samples but no amplification of untreated RNA samples. The marker is a 100 bp DNA ladder from Invitrogen.