Increased ambient temperature reduces grain yield in Brachypodium. (a,b) Fresh weight (a) and dry weight (b) of developing grain from plants grown at either 22/17°C (black line) or 27/22°C (red line) (day/night temperatures). Data are the replicate of 5 replicate plants, including measurements of at least 20 seed. Values are the mean ± standard error (**P < 0.01). (c) Final yield measurements in units of weight per 10 grain for plants transferred from 22/17°C to 27/22°C throughout grain-filling, then transferred back to 22/17°C at 16 days after pollination, compared to plants grown constantly at 22/17°C (***P < 0.001). Data are from 20 biological repeats. Values are the mean ± standard error.