Figure 5

H3K27me3 loss in hypomethylated cells is associated with ectopic gene expression. (A, B) Boxplots displaying the relative expression (log2) of H3K27me3 down genes (red) and all genes (white) between Dnmt1^+/+ and Dnmt1^-/- MEFs as measured by mRNA-seq (A) and expression microarray (B). **P <0.001 by Wilcoxon rank sum test. (C) Boxplot showing the difference in promoter enrichment for H3K4me3 between Dnmt1^+/+ and Dnmt1^-/- MEFs as measured by ChIP-chip. **P <0.001 by Wilcoxon rank sum test. (D) Venn diagram showing overlap between H3K27me3 down genes (red circle) and genes that show >2-fold increased expression in Dnmt1^-/- relative to Dnmt1^+/+ MEFs by mRNA-seq (yellow circle). The number of genes in each section of the diagram is shown. **P <0.001 as estimated by hyper-geometric testing for the enrichment of the overlap genes.