Figure 7
Brachypodium PARE data demonstrate miRNA-guided target cleavage. Examples of degradation plots for miRNA targets for each prominence level. The abundance of all the PARE sequences matching miRNA targets are plotted with blank diamonds. The PARE sequence corresponding to the miRNA-mediated cleavage is represented by a black diamond. (a) Bradi1g52240, encoding a GIBBERELLIC-ACID INSENSITIVE (GAI), REPRESSOR of GAI (RGA), and SCARECROW (SCR) (GRAS) transcription factor, is a Level 4 target of Bdi-miR171bcef. (b) Bradi3g05510, encoding a SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factor is a Level 3 target of Bdi-miR156b-ij. (c) Bradi4g39330, encoding a laccase, is a Level 2 target of Bdi-miR397ac. (d) Bradi3g51077, encoding argonaute 1a, is a Level 1 target of Bdi-miR168. nt, nucleotide; miRNA, microRNA; PARE, parallel analysis of RNA ends; TP10M, transcripts per 10 million.