Figure 3

H3.3 nucleosomes display fast and slow turnover rates. (A) Genome browser view of HA-H3.3 profiles at several genomic regions over a 6-hour time frame. Examples of HA-H3.3 peaks that exhibit slow (left two panels), intermediate (center two panels) and fast turnover (right two panels) are displayed. (B) Dot plot of turnover indices calculated from two independent experiments. (C) Heat map showing relative HA-H3.3 enrichment (red = low, green = high) across all time points sorted by turnover index from low to high. (D) Distribution of turnover indices reveals two populations of H3.3 nucleosome exchange.