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Figure 5 | Genome Biology

Figure 5

From: Regulation of transcriptome, translation, and proteome in response to environmental stress in fission yeast

Figure 5

mRNA, translation, and protein regulation during oxidative stress. (a) Hierarchical cluster analysis with columns representing experimental time points and rows representing 811 genes whose mRNAs showed significant expression changes after exposure to H2O2 and whose proteins could be detected in all conditions. mRNA1: mRNA expression relative to the unstressed samples is color coded as indicated at the bottom, using same samples as for the translation experiment (Figure 2). Translation: translational efficiency relative to the unstressed samples is color coded as indicated at the bottom. mRNA2: mRNA expression relative to the unstressed samples is color coded as indicated at the bottom, using the same samples as for the proteome experiment. Protein: protein expression relative to the unstressed samples is color coded as indicated at the bottom. Average data of biological repeats are shown, with missing data in gray. (b) Cluster analysis as in (a) for the 232 genes that encode proteins showing significant changes in expression in the proteome experiment and with data in >50% of all conditions used for clustering. (c) Scatter plot showing linear regressions of the maximum average log2 changes in mRNA and protein expression across the time-course experiment mRNA2/Protein shown in (a) for proteins that were detected in all conditions. Yellow dots, 193 genes showing >1.5-fold induction in mRNA expression after exposure to H2O2 in at least 4 of 7 stress time points in experiments mRNA1 and mRNA2 shown in (a); blue dots, 226 genes showing >1.5-fold repression in mRNA expression after exposure to H2O2 in at least 4 of 7 stress time points in experiments mRNA1 and mRNA2. Pearson's correlations and probabilities (two-tailed test) are indicated for induced (yellow) and repressed (blue) mRNAs. (d) Graph showing average mRNA (green) and protein (red) expression profiles (log2 ratios) in the experiment mRNA2/Protein shown in (a). Solid and dashed lines indicate average profiles for 193 and 226 genes showing >1.5-fold induction or repression, respectively, in mRNA expression after exposure to H2O2 in at least 4 of 7 stress timepoints in experiments mRNA1 and mRNA2 shown in (a).

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