Figure 2

Hog1 associates with RNA Pol II and RNA Pol III loci upon stress. (a) Stacked bars representing the percentage of Hog1-occupied ORFs upon stress as determined by ChIP-Seq data for Hog1-dependent and -independent subsets of genes (Z-score 4; P = 0.0001), as in Figure 1d). (b) Boxplot of Hog1 association upon stress (Z-score) at promoters (from -500 to the transcription start site) and ORFs in genes whose expression is classified as Hog1-dependent or Hog1-independent. (c) Hog1 recruitment at RNA Pol III loci of SCR1, RPR1 and tF(GAA)D. Histograms represent Hog1 ChIP-Seq profiles from a non-stressed sample normalized to that obtained upon osmostress. Right-hand panel: Validation of Hog1 recruitment to novel Hog1 sites by ChIP. (d) In vivo binding of Hog1 and RNA Pol III. Hog1-HA and a specific subunit of RNA Pol III (Rpc82-Myc) were expressed from their endogenous locus. Samples were taken before (-) and after (+) stress (0.4 M NaCl, 10 minutes).