Figure 8
From: Improving RNA-Seq expression estimates by correcting for fragment bias
Bias in different sequence technologies. Results of correlation tests showing improvement after bias correction of datasets generated using different sequencing technologies. The Illumina dataset is SRA012427 (x-axes) and the SOLiD data is SOLiD4_ HBR_PE_50x25 (y-axes). Both used the same MAQC HBR sample. Red axes and lines denote uncorrected FPKM values and blue corrected, while purple regression lines denote a comparison between corrected and uncorrected values. Both datasets are being corrected for different biases, which causes their expression estimates to become more correlated. Note that the plot is zoomed in on the lower abundance transcripts for clarity but captures over 98% of those in the experiment.