Variable library preparations. Results of correlation tests showing improvement after bias correction of datasets generated using different library prep methods, all of which are strand-specific. The first four protocols are described in  and the final in . All datasets were compared against a control that was generated using the standard Illumina RH protocol. The first four datasets used the control from  with the same yeast sample. The last dataset (NSR) was compared against the HBR dataset from SRA010153 since it is also consists of single-end reads.