Correlation between RNA-Seq and qRT-PCR. (a) Expression estimates before bias correction (tail of arrows) and after correction (points of arrows) for the SRA012427 dataset compared to qRT-PCR values for the same transcripts. Red arrows show decrease in expression after correction and blue an increase. Note that we have zoomed in on lower-expression transcripts (the majority) for clarity. (b) Distribution of log-fold change in expression after bias correction.