Figure 9

Differential gene expression by C. militaris in association with fruiting structure formation or growth in a liquid medium. (a) Estimation of significantly up- and down-regulated genes between different samples. (b) Heat map of protein kinases associated with the mitogen-activated and cAMP-dependent protein kinase pathways at different developmental stages. (c) Heat map of the highly expressed transcription factors at different developmental stages. Genes with expression values > 100 transcripts per million tags (TPM) are also indicated in red. Annotation information for the genes is provided in Table S19 in Additional file 1. DEG, differentially expressed gene. FB1, FB2 and FB3 are associated with nascent, stalk formation and mature developmental stages shown in Figure 5a-c, respectively. The transcriptome of undifferentiated mycelia harvested from SDB was included as a reference for gene expression analysis.