High-throughput RNA interference screening using pooled shRNA libraries and next generation sequencing

Table 1 Detection of depleted hairpins in reference depletion screens

The false positive and false negative rates at a range of log ratio thresholds were calculated by comparing each depletion group to the non-depleted hairpins. This was repeated for all hairpins and after filtering to exclude hairpins with low representation (<100 raw reads) in the reference set. The minimum false positive rate (Min FPR) found is shown along with the associated false negative rate (FNR).

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