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Table 3 Performance comparison of manual versus automated solution hybrid selection

From: A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries

Factor

Column based

Automated (with-bead SPRI)

Automated (with-bead SPRI) low input

Input DNA

3 μg

3 μg

0.1 μg

Samples/FTE/week

6-12

384

384

Number of sample transfer steps

10

4

4

Output DNA prior to PCR

720 ng

1,330 ng

Below limit of detection

Number of pond PCR cycles

12-16

6

6

Percentage duplicated reads

19.8

2.2

10

Percentage selected bases

84.7

88.6

83.76

Estimated library size

43 million

516 million

223 million

  1. FTE, Full time employee; SHS, solution hybrid selection; SPRI, solid-phase reversible immobilization.