Factor | Column based | Automated (with-bead SPRI) | Automated (with-bead SPRI) low input |
---|---|---|---|
Input DNA | 3 μg | 3 μg | 0.1 μg |
Samples/FTE/week | 6-12 | 384 | 384 |
Number of sample transfer steps | 10 | 4 | 4 |
Output DNA prior to PCR | 720 ng | 1,330 ng | Below limit of detection |
Number of pond PCR cycles | 12-16 | 6 | 6 |
Percentage duplicated reads | 19.8 | 2.2 | 10 |
Percentage selected bases | 84.7 | 88.6 | 83.76 |
Estimated library size | 43 million | 516 million | 223 million |