Strand-specific RNA sequencing reveals extensive regulated long antisense transcripts that are conserved across yeast species

Table 1 Strains and growth conditions

Strain number	Species	Background	Genotype	Source
BB32	Saccharomyces cerevisiae			Gift from Leonid Kruglyak's lab
BY4741	Saccharomyces cerevisiae	S288c	MATa, his3Δ1, leu2Δ0, met15Δ0, ura3Δ0	Gift from Andrew Murray's lab
	Saccharomyces cerevisiae	BY4741	Same as above with rrp6Δ::KANMX6	ATCC
	Saccharomyces cerevisiae	BY4741	Same as above with hda2Δ::URA3	Gift from Oliver Rando's lab
	Saccharomyces cerevisiae	BY4741	Same as above with rrp6Δ::KANMX6, hda2Δ::NatMX4	This study
NCYC2600	Saccharomyces paradoxus			NCYC Stock Center
IFO 1815	Saccharomyces mikatae			ATCC
CLIB 592	Saccharomyces castellii			CLIB Stock Center
CLIB 209	Kluyveromyces lactis			CLIB Stock Center

ISSN: 1474-760X