Deficiency in mouse Y chromosome long arm gene complement is associated with sperm DNA damage

Table 2 Western blot analysis of protamines in mice with NPYq deficiencies

	Mean band intensity^b (±SEM) representing mature protamine forms
Mice examined^a	Coomassie	Hub2B (protamine 2)	Mice examined^a	Mean band intensity^b (±SEM) representing immature protamine 2 (PreP2)
2/3NPYq- (n = 7)	141.9 ± 4.6	125.3 ± 4.6	-	-
XY^RIII (n = 6)	138.2 ± 5.0	124.9 ± 5.0
9/10NPYq- (n = 7)	82.5 ± 4.8*	43.5 ± 0.3***	9/10NPYq- (n = 5)	89.2 ± 4.5**
XY^Tdym1Sry (n = 6)	131.0 ± 5.2	49.3 ± 0.3	XY^Tdym1Sry (n = 4)	53.8 ± 5.0
NPYq-² (n = 3)	22.1 ± 16.2	20.8 ± 6.6
XY^RIII (n = 3)	76.0 ± 16.2	36.9 ± 6.6	-	-

^aAt least two separate gels were run for each NPYq deficient genotype (2/3NPY-, two gels; 9/10NPYq-, four gels; NPYq-², two gels). For PreP2, data were obtained from only two gels. ^bThe data were analyzed by two-way ANOVA with gel and genotype as factors, from which the genotype means and errors are derived. Statistical significance: *P < 0.005; **P < 0.0005; ***P < 0.00005. The lack of statistical significance for the reduction observed in NPYq-² was almost certainly due to the limited number of samples. SEM, standard error of the mean.

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