Figure 4

Oocyte arrest after ICSI with sperm from mice with NPY deficiency. Epididymal sperm from males with severe NPYq deficiency (9/10NPYq- and NPYq-²) but not males with moderate NPYq gene loss (2/3NPYq-) led to increased incidence of oocyte arrest compared to epididymal sperm from their appropriate controls, as revealed by Fisher's exact probability test. Statistical significance: a = P < 0.05; c = P < 0.001 versus matching sperm type in control. Epididymal sperm from males with severe NPYq deficiency (9/10NPYq- and NPYq-²) but not males with moderate NPYq gene loss (2/3NPYq-) led to increased incidence of oocyte arrest compared to testicular sperm, as revealed by Mantel-Haenszel chi square analysis. Statistical significance: **P < 0.01; ***P < 0.001. Two males were used per genotype; four sperm groups (epididymal, frozen epididymal, testicular and frozen testicular) were examined per male; approximately 20 (20.35 ± 6.94) oocytes were scored per group per male.