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Figure 5 | Genome Biology

Figure 5

From: Detection and analysis of alternative splicing in Yarrowia lipolytica reveal structural constraints facilitating nonsense-mediated decay of intron-retaining transcripts

Figure 5

Schematic diagram of alternative variants of YALI0D18403g. The two different transcription start sites (TSS1 and TSS2) are indicated by arrows. (a) TSS2 is located 179 bases upstream of the methionine initiation codon of YALI0D18403g1 (position 2309045 on chromosome D) downstream of YALI0D18436g and allows the transcription of a single exon. Translation of this mRNA generates a putative protein of 1,322 amino acids. (b) TSS1 is located about 3 kb upstream of TSS2 and initiates a transcript with a 3,478-nucleotide intron. Surprisingly, this intron overlaps YALI0D18436g, a CDS of 1,062 bases the translation of which generates a putative 353 amino acid protein of unknown function. Translation of the YALI0D18403g2 mRNAs generates a putative protein of 1,424 amino acids.

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