Figure 4

Profiling of mutants hypersensitive to a microtubule-depolymerizing drug and three genotoxic agents. The mutant pools grown in YES medium were treated with thiabendazole (TBZ), camptothecin (CPT), hydroxyurea (HU), and UV radiation. Three independent experiments, called A, B, and C, were conducted with an untreated control sample included in each experiment. The treatment doses were the same for experiments A and B, while in experiment C the doses were doubled. (a) The growth inhibition scores (GI) of control versus 50 J/m² UV treatment (experiment UV_A). Strains with GI values > 0.5 are highlighted in red. (b) Genes with high GI values in experiment UV_A are more strongly associated with the GO annotation of DNA damage response (DDR) genes. The 83 genes whose GI ≥0.15 in experiment UV_A are classified according to whether they are associated with the GO term 'response to DNA damage stimulus' [GO:0006974]. (c) Genes with high GI values in experiment UV_A are more likely to be identified as hypersensitive hits by surpassing the GI and P-value cutoffs more than once in three independent experiments. The 83 genes whose GI ≥0.15 in experiment UV_A are classified according to whether they are selected as hypersensitive hits. (d) The GO terms most highly enriched among the hypersensitive mutants identified by barcode sequencing. (e) Hierarchical clustering analysis of the hypersensitive mutants identified by barcode sequencing. For a detailed view of the heat map, see Additional file 18.