Table 1 Comparison of decay rates and half-lives of 17 selected genes determined from microarray data and qRT-PCR
From: Short RNA half-lives in the slow-growing marine cyanobacterium Prochlorococcus
| Â | Microarray | qRT-PCR | ||||
|---|---|---|---|---|---|---|
Gene | Cluster | Expression at time 0 [log2] | Half-life [min] | Decay rate [min] | Half-life [min] | Decay rate [min] |
PMM1077 | 7 | 5.6 | 1.7 | 2.4 | 1.6 | 1.6 |
dnaN | 3 | 6.4 | 1.5 | 1.7 | 3.0 | 2.1 |
psaK | 9 | 12.3 | 4.9 | 5.0 | 5.3 | 4.8 |
atpA | 11 | 10.9 | 12.2 | 4.9 | 6.2 | 3.3 |
psbA | 11 | 14.3 | 40.1 | 71.0 | 18.5 | 16.2 |
recN | ND | 4.0 | 3.9 | 5.3 | 2.2 | 6.4 |
recA | 5 | 8.5 | 2.3 | 2.7 | 2.6 | 7.5 |
ftsZ | 5 | 9.4 | 1.8 | 2.1 | 3.4 | 2.1 |
amt1 | 11 | 13.7 | 52.1 | 77.2 | 17.3 | 11.3 |
psbD | 9 | 13.2 | 9.0 | 8.8 | 7.0 | 5.6 |
PMM1121 (som) | 11 | 13.9 | 28.6 | 39.1 | 13.0 | 10.4 |
pcb | 11 | 13.9 | 15.6 | 25.8 | 6.6 | 6.3 |
PMM1447 | ND | 3.7 | 59.5 | 18.0 | 40.6 | 4.1 |
atpE | 11 | 13.0 | 13.5 | 24.8 | 15.6 | 4.7 |
atpB | 9 | 10.5 | 4.6 | 3.4 | 8.0 | 4.7 |
atp1 | 10 | 10.7 | 5.0 | 2.3 | 2.4 | 2.6 |
16S rRNA | 2 | 14.9 | 370.1 | 20.7 | -261.3 | 54.4 |