Skip to main content
Figure 8 | Genome Biology

Figure 8

From: Rapid chromosome territory relocation by nuclear motor activity in response to serum removal in primary human fibroblasts

Figure 8

Suppression of nuclear myosin expression by short interference RNAs (siRNAs). Normal human dermal fibroblasts (HDFs) were transfected with negative control or MYO1C targeting siRNA (double transfection) and samples for immunofluorescence staining and 2D-FISH (fluorescence in situ hybridization) were fixed 48 hours after the final transfection. Representative images of nuclei stained for anti-NMIβ (red) in control (g, h, m, n) cells transfected with negative control siRNA (i, j, o, p) and in cells transfected with MYO1C siRNA (k, l, q, r) after 0 and 15 minutes of serum starvation are displayed. The percentage of nuclei that are positive for NM1β in controls, in cells transfected with negative control siRNA, and in cells transfected with MYO1C siRNA are displayed in the adjacent table (s).

Back to article page