Figure 10

Anti-nuclear myosin 1b (NM1β) staining patterns in proliferating cells, quiescent cells, and after restimulation. Normal 2DD human dermal fibroblasts (HDFs) were serum starved for 7 days to induce quiescence. The cells were then restimulated with fresh serum, and samples were collected at 0, 24, 36 and 48 hours after serum restoration. Samples were also collected before serum withdrawal (proliferating cells). The samples were then fixed with methanol/acetone (1:1), and the distribution of NMIβ was assessed by performing an indirect immunofluorescence assay for NMIβ. Images in (a, c) display the distribution of NMIβ in proliferating cells, whereas those in (d and f) show the distribution of NMIβ after 0, 24, 36 and 48 hours after restimulation of quiescent fibroblasts. The table (p) displays the percentage of cells displaying various patterns of NMIβ staining after restimulation. Error is indicated by standard deviation. Scale bar = 10 μm.