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Table 1 Velvet assemblies

From: De novo genome sequence assembly of a filamentous fungus using Sanger, 454 and Illumina sequence data

ID

T42

T38

T36

T36; QRL(Q10) = 28

Total contigs

6,945

8,637

19,118

39,488

N50 contig

24,566 (N/A)

10,706

2,902

1,299

Total DNA (bp)

26,721,397

26,466,756

25,854,719

24,812,690

EST analysis*

6,585/29

6,204/24

4,657/11

2,923/9

  1. *EST alignments are given as: Complete alignments/Misassemblies (see Materials and methods). Velvet assemblies were generated from Illumina GAii read data. Assembly T42 was generated from the untrimmed, no-call and shadow filtered Illumina PE reads. Assemblies T38 and T36 were generated by trimming the last 4 and 6 bp, respectively, from the T42 read set. Assembly T36, QRL(Q10) = 28 was generated with the T36 read set from which reads were removed if they failed the QRL(Q10) = 28 quality region length filtering (see Materials and methods).