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Figure 4 | Genome Biology

Figure 4

From: De novo genome sequence assembly of a filamentous fungus using Sanger, 454 and Illumina sequence data

Figure 4

Assessing the discovery of unique read information between the Illumina and 454 platforms. (a) Raw reads were processed into overlapping 28-bp k-mers, and any k-mer that varied from all other k-mers by at least 1 bp was accepted as new sequence information. The analysis was done separately for unique k-mers and those that occurred at least twice (2× k-mers). (b) MAQ was then used to map these k-mers to the reference genome sequence and the rate at which new coverage was generated was plotted against the number of k-mers examined.

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