Figure 3
From: Identification and characterization of novel amphioxus microRNAs by Solexa sequencing
Confirmation of the accuracy of Solexa sequencing with qRT-PCR and microarray analysis. (a) The expression levels of the indicated miRNAs in amphioxus evaluated by semi-quantitative RT-PCR with 30 cycles. (b-d) Nine miRNAs (bbe-miR-1, bbe-miR-10a, bbe-miR-29b, bbe-miR-92a, bbe-miR-125, bbe-miR-184, bbe-miR-210, bbe-miR-216, and bbe-miR-217) were selected and their expression levels were measured by Solexa sequencing, stem-loop qRT-PCR and microarray analysis. The data obtained from each of these methods were then compared with the data obtained from each of the others and drawn as a Pearson correlation scatter plot.