Figure 1

Reproducibility of detections in the mixture data. Reproducibility of the probe-level PECA-SI, the standard SI procedures RMA-SI, PLIER-SI, RMA-MIDAS and PLIER-MIDAS, the two-way ANOVA-based approaches RMA-LM and PLIER-LM, and the FIRMA algorithm in detecting differential splicing in the mixture data. The ability of the methods to reproduce the detections from the pure brain and heart samples was studied at various levels of the mixture differences (x-axis). The reproducibility was measured as the overlap of the top-ranked 500 detections between the mixture and pure datasets. At each mixture difference, the same data were analyzed with the different detection methods. Reproducibility in random data is shown as a reference (0.002). Similar results were produced with the top-ranked 100, 1,000, 1,500 and 2,000 detections [see Additional data file 1].