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Table 1 Proteins in honeybee queen spermathecal fluid

From: Insights into female sperm storage from the spermathecal fluid proteome of the honeybee Apis mellifera

 

Mated

Virgin

    

PreRelease2 accession

Gel

LC MS/MS

LC MS/MS

Seminal fluid

Sperm

Pred. secret.

Protein functional description

GB10467-PA

X

X

 

X

  

Aspartate aminotransferase 2 precursor

GB10973-PA

 

X

X

X

  

Arginine kinase

GB15049-PA

  

X

   

Delta-1-pyrroline-5-carboxylate synthetase

GB15171-PA

X

X

  

X

 

Ornithine aminotransferase precursor

GB16218-PA

  

X

   

Proline oxidase

GB17641-PA

X

X

X

   

Alanine aminotransferase 2

GB18844-PA

X

X

X

   

Glutamate oxaloacetate transaminase 1

GB10133-PA

X

X

    

Superoxide dismutase

GB10498-PA

X

X

X

   

Peroxiredoxin

GB12029-PA

X

     

Glyoxalase domain-containing protein

GB14972-PA

X

    

S

Thioredoxin reductase

GB15855-PA

X

     

Thioredoxin-2

GB18955-PA

 

X

X

X

 

S

Phospholipid hydroperoxide glutathione peroxidase

GB19380-PA

 

X

X

   

Thioredoxin peroxidase 1

GB30268-PA

X

X

X

   

Glutathione s transferase S1

GB12586-PA

  

X

  

S

Protein disulfide-isomerase precursor

GB12447-PA

X

     

CAP, adenylate cyclase-associated protein 1

GB13596-PA

  

X

   

ATP synthase

GB14791-PA

  

X

   

ATP synthase subunit

GB15291-PA

  

X

   

ATP synthase gamma subunit

GB16485-PA

  

X

   

ATP synthase D chain

GB10989-PA

X

X

X

   

Vacuolar ATPase catalytic subunit A

GB11380-PA

X

X

X

   

Vacuolar H+ ATPase 44 kDa C subunit

GB12913-PA

X

X

X

   

Vacuolar proton pump E subunit

GB13499-PA

X

X

X

   

Vacuolar ATPase subunit G

GB15226-PA

X

X

    

Vacuolar ATPase subunit D 1

GB17480-PA

X

X

X

   

Vacuolar ATPase subunit H

GB17499-PA

  

X

   

ADP/ATP translocase

GB19171-PA

X

X

X

   

Vacuolar ATPase 55 kDa B subunit

GB20017-PA

X

     

Endoplasmic reticulum ATPase

GB10355-PA

 

X

X

  

S

Melittin

GB10695-PA

X

X

X

 

X

 

Pyruvate kinase

GB10992-PA

X

     

ATP citrate lyase isoform A

GB11056-PA

X

X

X

X

  

Phosphoglycerate kinase isoform 1

GB11461-PA

X

     

UTP-glucose-1-phosphate uridylyltransferase

GB12488-PA

  

X

   

Aconitase

GB12573-PA

 

X

X

X

  

Citrate synthase

GB12741-PA

  

X

   

Aldehyde dehydrogenase

GB12949-PA

 

X

X

   

6-Phosphogluconate dehydrogenase

GB13058-PA

 

X

  

X

 

Dihydroxyacetone kinase 2

GB13237-PA

X

X

X

   

Phosphogluconate mutase

GB13882-PA

X

X

    

L-lactate dehydrogenase

GB13955-PA

 

X

X

   

N-acetyltransferase 5

GB14517-PA

 

X

X

   

Isocitrate dehydrogenase

GB14798-PA

X

X

X

 

X

 

Glyceraldehyde-3-phosphate dehydrogenase 2

GB14803-PA

 

X

X

  

S

Alpha,alpha-trehalase

GB15039-PA

X

X

X

   

Enolase

GB15052-PA

X

X

X

   

Phosphoglyceromutase

GB15463-PA

 

X

  

X

 

Aldolase

GB15543-PA

 

X

 

X

  

Malate/L-lactate dehydrogenases

GB15619-PA

X

     

Transketolase-like

GB15888-PA

 

X

X

   

Carbonic anhydrase

GB16429-PA

X

X

X

X

  

Glucose-6-phosphate isomerase

GB16464-PA

X

X

X

   

malate dehydrogenase

GB16951-PA

X

     

Malic enzyme

GB17113-PA

X

X

X

   

Phosphofructokinase

GB17473-PA

X

X

X

X

  

Triosephosphate isomerase 1

GB18109-PA

 

X

  

X

 

Aldose reductase (NADP+)

GB18727-PA

X

X

X

   

Malate dehydrogenase

GB19030-PA

X

X

X

   

Aldo/keto reductase family protein

GB19387-PA

 

X

X

 

X

 

Hexokinase A, isoform A

GB19460-PA

X

X

X

   

Aldolase, isoform F

GB11665-PA

 

X

X

X

 

S

Chitinase-like protein

GB11876-PA

  

X

  

S

LDLa domain containing chitin binding protein

GB16986-PA

 

X

X

  

S

Endochitinase precursor

GB10397-PA

  

X

   

Alpha-crystallin

GB10800-PA

X

     

T-complex protein 1

GB10836-PA

X

     

HSP70

GB14758-PA

X

X

X

X

  

Heat shock protein 90

GB14852-PA

X

X

X

X

  

Heat shock protein 8 isoform 1

GB15016-PA

X

X

X

   

Heat shock protein cognate 3

GB17056-PA

X

X

X

   

Cyclophilin 1

GB18662-PA

  

X

   

Alpha-crystallin, small HSP

GB18969-PA

X

 

X

   

Heat shock protein 60

GB12818-PA

  

X

   

Histone 2A

GB14548-PA

 

X

X

  

S

Deoxyribonuclease II

GB16515-PA

X

     

ATP dependent DNA helicase

GB19247-PA

X

     

Elongation factor 2, isoform 1

GB16568-PA

  

X

X

  

Cytochrome c oxidase subunit

GB19293-PA

X

X

X

   

Cytochrome c

GB19729-PA

X

X

   

S

Cytochrome c

GB11059-PA

X

     

Retinoid- and fatty-acid binding protein

GB15044-PA

 

X

X

X

  

Phosphatidylethanolamine-binding protein

GB14639-PA

 

X

X

  

S

Major royal jelly protein 8

GB16324-PA

 

X

X

  

S

Major royal jelly protein 9

GB12951-PC

X

X

X

   

14-3-3-like protein

GB15202-PA

 

X

    

DJ-1, neuroprotective transcriptional co-activator

GB15582-PA

 

X

X

   

14-3-3 epsilon

GB16178-PA

X

     

Neuropeptide Y receptor

GB16716-PA

  

X

  

S

Leucine-rich repeat-containing protein

GB16072-PA

X

X

X

   

Iron regulatory protein 1B

GB10536-PA

  

X

   

Odorant binding protein 14

GB19662-PA

 

X

   

S

Juvenile hormone binding protein

GB19745-PA

 

X

   

S

Transferrin

GB10009-PA

X

X

X

 

X

 

Tubulin alpha-1 chain

GB10091-PA

  

X

  

S

Cuticlin-1 precursor

GB10122-PA

 

X

X

 

X

 

Tubulin, beta, 2

GB10275-PA

X

X

X

X

  

Tubulin isoform B

GB10514-PA

X

X

X

 

X

 

alpha tubulin

GB11282-PA

X

X

X

   

Moesin isoform D

GB11920-PA

X

     

Tubulin

GB12614-PA

 

X

    

Actin

GB13049-PA

 

X

    

Tubulin, beta, 2

GB13229-PA

X

     

PDZ and LIM domain protein

GB13999-PA

X

X

X

  

S

Vitellogenin

GB15794-PA

  

X

  

S

Cuticlin-1 precursor

GB16448-PA

 

X

X

X

  

Annexin IX

GB17673-PA

X

     

Talin-1

GB17681-PA

X

X

X

X

  

Actin-5C isoform 1

GB18917-PA

 

X

X

   

Cofilin/actin-depolymerizing factor homolog

GB12113-PA

  

X

   

Porin

GB14012-PA

  

X

   

Phosphate carrier

GB16577-PA

  

X

   

Sialin, inorganic phosphate cotransporter

GB11987-PA

 

X

 

X

  

Unknown

GB12562-PA

X

X

X

   

Hypothetical protein

GB13778-PA

X

    

S

Unknown

GB14970-PA

X

     

Muscle-specific protein 300

GB15662-PA

X

X

X

X

  

Unknown

GB17311-PA

 

X

X

X

 

S

Unknown

GB17500-PA

  

X

  

S

Hypothetical protein

GB19255-PA

X

    

S

Osiris 14 CG1155-PA

GB30569-PA

 

X

X

  

S

Hypothetical protein

  1. Proteins were identified by MS/MS analysis of one-dimensional gel and gel-free analysis of tryptic peptides from spermathecal fluid extracted from mated and virgin queens. Data are compared to the previous reported analysis of the male seminal fluid and sperm proteomes [19]. Details of the mass spectrometry analysis and detailed matching are shown in Additional data file 1. Significant identification of peptides from a specific bee locus number are shown with an 'X' for gel analyses; the number of proteins predicted to be secreted (Pred. secret.) are shown with an 'S' based on consensus of analysis of amino acid sequences using two different software packages TargetP [52] and IPsort [53].