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Figure 5 | Genome Biology

Figure 5

From: Chætognath transcriptome reveals ancestral and unique features among bilaterians

Figure 5

Alternative forms of selected markers amplified by PCR in order to assess the origin of polymorphism. (a) Localization of sperm within sperm receptacles (SR) and sperm ducts (SD) in the body of chætognath S. cephaloptera along with ovaries (Ov) and testis (Te). The double arrow indicates that head and body of individuals were split to perform independent PCR amplifications with the purpose of detecting possible contamination from the sperm genome. (b) Paralogous copies of nuclear genes RP L36 and L40 with their intron positions and average lengths, which are distinct in both cases (Additional data files 5 and 6). The names and positions of primers used for the amplification are also specified (Table S3 in Additional data file 2). (c) Relationships between alternative copies of Cytb retrieved within the ESTs with the three different forms detected by the designed primers (Additional data file 8). Boostrap proportions are indicated for selected nodes.

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