Figure 7

Vegetative growth of Mgd1 deletion mutants on various nutrient sources. (a-h) Wild type 70-15 (a-d) and Mgd1 deletion mutant (e-h) were grown on minimal media (a,e), complete media (b,f), minimal media with Tween 20 (c,g) or polyethylene glycol (d,h) as carbon source for seven days. Results shown are typical of all four independent Mgd1 deletion mutants. Results for ectopic strains were similar to wild type (data not shown). (i-k) Mgd1 deleted mutants (ΔMgd1a, ΔMgd1b), ectopic strains (ectopic a, ectopic b) and wild type 70-15 (WT) were grown for seven days on minimal media (0.125% glucose) with glutamine (i) and glutamic acid (j) as nitrogen source and minimal media (1% glucose) (k) as depicted in (l). Photographs in (i-k) were taken on a light box to highlight differences in mycelial density.