Gene expression profiling of human prostate cancer stem cells reveals a pro-inflammatory phenotype and the importance of extracellular matrix interactions

Table 2 Summary of patient population and invasive characteristics of corresponding stem cell cultures in vitro

Patient number	Age (years)	Origin	Gleason score	% Invasion in vitro*
228	-	LN metastasis	7	101 ± 21
434	59	Prostate	8/9	99 ± 56
484	69	Prostate	7	105 ± 29
512	74	Prostate	BPH	-
561	72	Prostate	BPH	-
563	64	Prostate	7	35 ± 9.5
569	64	Prostate	8	75 ± 9
574	74	Prostate	BPH/G6 (5%)	-
605	56	LN metastasis	7	119 ± 21
661	78	Prostate	6	-
627	79	Prostate	BPH	-
662	66	Prostate	BPH	-
665	53	Prostate	7	63 ± 18.4
667	47	Prostate	6	61 ± 4.2
687	63	Prostate	7	74
690	79	Prostate	BPH	-
693	75	Prostate	BPH	-
704	64	Prostate	7 (HR)	-
003/06	52	Prostate	6	-

*Invasion assays were carried out on total epithelial cell populations before fractionation according to [4]. Positive controls for invasive capacity were cell lines MCF7 and PC3M whose invasion score was 18-36%, whereas normal cell lines PNT2 and PNT1a and BPH/primary normal prostate invasion scores ranged from 3-6%. Patient 704 was being treated (hormone refractory (HR)) by androgen blockade therapy.

ISSN: 1474-760X