- Open Access
RNA polymerase II stalling: loading at the start prepares genes for a sprint
© BioMed Central Ltd 2008
- Published: 02 May 2008
Stalling of RNA polymerase II near the promoter has recently been found to be much more common than previously thought. Genome-wide surveys of the phenomenon suggest that it is likely to be a rate-limiting control on gene activation that poises developmental and stimulus-responsive genes for prompt expression when inducing signals are received.
- Histone Mark
- IMR90 Cell
- Transcriptional Elongation
- Prompt Expression
- General Transcription Factor TFIID
The recruitment of RNA polymerase II (Pol II) to the promoter has been generally believed to be the rate-limiting step in gene activation . However, a series of discoveries made since the mid-1980s, combined with recent genome-wide studies, suggest that many developmental and inducible Drosophila and mammalian genes, prior to their expression, contain Pol II bound predominantly in their promoter proximal regions in a 'stalled' state [1–8]. Activation of the stalled polymerase is thought to be responsible for the expression of these genes .
Distinct sets of accessory factors are associated with Pol II stalling and its escape from stalling, acting either by direct interaction with Pol II, or by manipulating the chromatin environment - for example, by affecting histone modifications by histone methyltransferases (HMTs) or histone acetyltransferases (HATs) . Proteins associated with Pol II stalling include the DRB sensitivity-inducing factor (DSIF) and the negative elongation factor (NELF) [9, 10], whereas proteins such as the positive transcription-elongation factor-b (P-TEFb) complex, and the general transcription factors TFIIS and TFIIF contribute to escape from stalling [11, 12]. These latter proteins enable Pol II to begin transcription elongation on induction by heat shock, for example. Thus, all the factors mentioned above may serve as a stalling checkpoint to poise genes for prompt expression.
Although initial studies revealed that Pol II stalling is present on several genes, it has recently been found that Pol II stalling is more common than previously thought [13–16]. Technologies such as ChIP-chip (chromatin immunoprecipitation in combination with genomic DNA microarrays) allow transcriptional regulation to be examined genome wide [17, 18]. Through the ENCODE (Encyclopedia of DNA Elements) project , in which 1% of the human genome has been extensively analyzed, and through genomic studies in Drosophila, human embryonic stem cells (hESCs) and other human cell lines, Pol II stalling is now seen as a genome-wide phenomenon. Moreover, it is enriched at highly regulated genes that are essential for responses to stimuli and for embryonic development [13, 14]. In addition, stalled Pol II signals are associated with active histone modification marks, including trimethylation of lysine 4 on histone H3 (H3K4me3) and acetylation of H3 lysine 9 and 14 (H3K9ac and H3K14ac) . Thus, Pol II promoter-proximal stalling could help to provide an active chromatin environment and prepare developmental and stimulus-responsive genes for timely expression [1, 20]. However, for a gene to proceed to transcriptional elongation, additional histone modifications are necessary . In this article, we review the mechanisms of Pol II stalling, with a focus on recent genomic and epigenomic findings, and discuss the biological implications of the widespread stalling phenomenon.
Pol II promoter-proximal stalling was first described in Drosophila heat-shock-inducible genes (for example, Hsp70) using ultraviolet-crosslinking and chromatin immunoprecipitation (UV ChIP), which captures the specific proteins and their bound DNA in vivo . Pol II was found to be recruited to the promoter of the uninduced Hsp70 gene, where it initiates RNA synthesis but stalls after synthesis of 20-50 nucleotides of RNA [1, 21]. Heat-shock stimulation enabled Pol II to escape from the Hsp70 promoter-proximal region and transcribe the full-length RNA. Thus, the regulation of Pol II stalling rather than of transcription initiation is rate-limiting for expression of this gene. After this initial discovery, Pol II stalling was observed in more than a dozen Drosophila and viral (HIV) genes, as well as in mammalian genes (Myc, Junb, and Fos), in studies using UV ChIP and nuclear run-on methods [1–8].
Pol II stalling was found to result from repression of transcript elongation by at least two protein complexes: DSIF and NELF [9, 10]. ChIP assays showed that both DSIF and NELF are co-localized with the stalled polymerase in the promoter-proximal regions of uninduced Drosophila heat-shock genes [9, 10]. The mechanisms by which DSIF and NELF regulate Pol II stalling are still under investigation. As NELF has been found to bind to RNA, it is possible that NELF exerts its repressive function through interaction with the nascent RNA .
Until very recently, Pol II stalling was only known at the promoters of a limited number of genes. Large-scale transcriptional regulation studies have enabled Pol II locations to be examined on a whole-genome scale. ChIP-chip experiments in both human and Drosophila using tiling oligo-nucleotide microarrays have demonstrated that Pol II stalling is a genome-wide phenomenon and more common than previously thought [17, 18]. Kim et al.  showed that, in addition to genes, such as Fos, that were known to have stalled Pol II, large numbers of promoters of human genes are bound by the Pol II preinitiation complex (PIC), although no expression was detected for these genes. These authors used specific antibodies against the PIC components of Pol II and the general transcription factor TFIID in ChIP-chip assays to map global PIC-binding sites in human primary fibroblast IMR90 cells. Promoter occupancy by the PIC was correlated with the genome-wide expression profiles of IMR90 cells. Kim et al. found that more than 600 genes were bound by the PIC but were not expressed. Obviously, regulatory mechanisms other than Pol II recruitment to the promoters are necessary to express these transcripts.
More recently, Muse et al.  reported a genome-wide search using ChIP-chip for Pol II promoter-proximal stalling in Drosophila. Among the genes bound by Pol II, some carry uniform Pol II binding throughout the gene, including the coding region, whereas in others Pol II binding signals were prominent in the promoter regions, and either absent or present at a low level within the full-length genes, consistent with the unique features of stalled Pol II. The genes with stalled Pol II showed low or no expression, whereas the uniformly bound genes showed a good correlation between Pol II occupancy and expression level. Other evidence supported the notion that the promoter-enriched binding was indeed due to Pol II stalling. Permanganate footprinting assays were carried out to test promoter melting by monitoring the reactivity of thymine residues. The hyper-reactivity of single-stranded thymine residues confirmed the presence of the stalled polymerase in the transcription bubble . In addition, NELF occupancy was also detected via ChIP at the genes with stalled Pol II. Depleting NELF by RNA interference significantly decreased Pol II signals in the promoter region only and not throughout the gene. Interestingly, Gene Ontology analysis revealed that the genes bound by stalled polymerase are enriched in developmental and stimulus-responsive genes involved in cell differentiation, cell-cell signaling and immune response pathways. Finally, it was shown that the stalled Pol II could be rapidly released upon gene induction, such as with UV irradiation .
Using similar methods, Zeitlinger et al.  reported a comprehensive Pol II ChIP-chip study in Drosophila embryos. This study took advantage of a mutant embryo that consists of mesodermal precursor cells alone. Neuronal- and ectodermal-specific genes are repressed in this mutant embryo. Pol II stalling was observed at more than 1,000 genes. In these cases, there is a much higher Pol II signal associated with the 5' region of the gene than the 3' region, and neuronal and ectodermal developmental genes were over-represented among the genes with stalled Pol II. On the other hand, ubiquitously expressed genes, such as genes for ribosomal components, exhibited uniform Pol II binding signal throughout the entire gene and a high level of expression. Zeitlinger et al.  also found genes that lacked Pol II binding altogether. These genes were enriched in genes for adult functions that do not need to be expressed in the embryo.
Recent studies of the transcriptome using microarrays or sequencing have disclosed large amounts of transcriptional activity throughout the human genome, with many of the transcripts being present as polyA+ RNA [18, 28–30]. The biological role of this 'unannotated' transcription, much of which is not expected to encode proteins, remains elusive. In particular, it was reported that short transcribed sequences of less than 200 nucleotides are clustered at the 5' and 3' ends of genes, producing the so-called promoter-associated sRNAs (PASRs) and termini-associated sRNAs (TASRs) . Intriguingly, the approximate lengths of one major class of PASRs are 26, 38 and 50 nucleotides, which is consistent with the lengths of the short transcripts reported to be produced as a result of Pol II stalling. However, there are usually multiple PASRs in the promoter region, not necessarily starting from the same site. Whether these represent multiple transcriptional start sites and the relationship between these PASRs and Pol II stalling are not yet clear. Finally, Pol II binding signals are also observed at the 3' ends of transcripts. It is possible that Pol II also pauses upon termination of transcription (Z Lian, A Karpikov, J Lian, MC Mahajan, S Hartman, M Gerstein, MS and SM Weissman, unpublished data). Further study is necessary to reveal the role of Pol II stalling in global transcription. The emerging technology of massively parallel sequencing will facilitate this effort [32–34].
What is the broad implication of Pol II stalling beyond the heat-shock response ? The genomic studies discussed in this review suggest that Pol II stalling is much more widespread than previously thought, and could serve as a rate-limiting step in transcriptional regulation to prepare organisms to respond to dynamic environmental and developmental changes. The prompt generation of gene products is crucial for the development and survival of the organism. Zeitlinger et al.  found that genes with stalled Pol II were highly enriched among developmental genes that are destined to be transcribed very soon - within 12 hours. Furthermore, Pol II promoter-proximal stalling could help to establish an active chromatin structure and allow prompt regulation of gene expression upon environmental stimuli and developmental signals. However, it is not clear how active histone marks are established around the 'poised' genes and whether the deposition of the histone marks depends on Pol II occupancy. Only about 70% of the genes marked by H3K4me3 or H3K9ac and K14ac are associated with Pol II promoter-proximal binding . How Pol II stalling is regulated also remains to be investigated. As noted above, data from Hsp70 indicate that stalling is likely to be mediated through a repressive mechanism. One possible developmental regulator of Pol II stalling may be the protein Snail, which represses mesodermal gene expression in Drosophila embryos [14, 35].
Further research is needed to identify additional factors that play important roles in Pol II stalling and the escape from stalling to transcriptional elongation. For example, the loss of key factors such as NELF and DSIF does not completely eliminate Pol II stalling. Also, there are indications from co-immunoprecipitation assays that additional elongation activators interact with the P-TEFb kinase, but the evidence is not conclusive . Lastly, the issue of how stimuli and developmental signals trigger the elongation machinery to release the stalled Pol II will be of prime interest for future investigations.
We thank Maya Kasowski, Wei Zheng, Karl Waern, and Christopher Hef-felfinger for critical reading of the manuscript and discussion. We acknowledge the members of the Snyder lab for help and support. JQW is supported by an NIH Ruth L Kirschstein National Research Service Award and an NIH training grant. MS and research in the Snyder laboratory is supported by grants from the NIH.
- Saunders A, Core LJ, Lis JT: Breaking barriers to transcription elongation. Nat Rev Mol Cell Biol. 2006, 7: 557-567. 10.1038/nrm1981.PubMedView ArticleGoogle Scholar
- Barboric M, Peterlin BM: A new paradigm in eukaryotic biology: HIV Tat and the control of transcriptional elongation. PLoS Biol. 2005, 3: e76-10.1371/journal.pbio.0030076.PubMedPubMed CentralView ArticleGoogle Scholar
- Aida M, Chen Y, Nakajima K, Yamaguchi Y, Wada T, Handa H: Transcriptional pausing caused by NELF plays a dual role in regulating immediate-early expression of the junB gene. Mol Cell Biol. 2006, 26: 6094-6104. 10.1128/MCB.02366-05.PubMedPubMed CentralView ArticleGoogle Scholar
- Gilmour DS, Lis JT: RNA polymerase II interacts with the promoter region of the noninduced hsp70 gene in Drosophila melanogaster cells. Mol Cell Biol. 1986, 6: 3984-3989.PubMedPubMed CentralView ArticleGoogle Scholar
- Bender TP, Thompson CB, Kuehl WM: Differential expression of c-myb mRNA in murine B lymphomas by a block to transcription elongation. Science. 1987, 237: 1473-1476. 10.1126/science.3498214.PubMedView ArticleGoogle Scholar
- Strobl LJ, Eick D: Hold back of RNA polymerase II at the transcription start site mediates down-regulation of c-myc in vivo. EMBO J. 1992, 11: 3307-3314.PubMedPubMed CentralGoogle Scholar
- Krumm A, Meulia T, Brunvand M, Groudine M: The block to transcriptional elongation within the human c-myc gene is determined in the promoter-proximal region. Genes Dev. 1992, 6: 2201-2213. 10.1101/gad.6.11.2201.PubMedView ArticleGoogle Scholar
- Sims RJ, Belotserkovskaya R, Reinberg D: Elongation by RNA polymerase II: the short and long of it. Genes Dev. 2004, 18: 2437-2468. 10.1101/gad.1235904.PubMedView ArticleGoogle Scholar
- Wu CH, Yamaguchi Y, Benjamin LR, Horvat-Gordon M, Washinsky J, Enerly E, Larsson J, Lambertsson A, Handa H, Gilmour D: NELF and DSIF cause promoter proximal pausing on the hsp70 promoter in Drosophila. Genes Dev. 2003, 17: 1402-1414. 10.1101/gad.1091403.PubMedPubMed CentralView ArticleGoogle Scholar
- Yamaguchi Y, Inukai N, Narita T, Wada T, Handa H: Evidence that negative elongation factor represses transcription elongation through binding to a DRB sensitivity-inducing factor/RNA polymerase II complex and RNA. Mol Cell Biol. 2002, 22: 2918-2927. 10.1128/MCB.22.9.2918-2927.2002.PubMedPubMed CentralView ArticleGoogle Scholar
- Peterlin BM, Price DH: Controlling the elongation phase of transcription with P-TEFb. Mol Cell. 2006, 23: 297-305. 10.1016/j.molcel.2006.06.014.PubMedView ArticleGoogle Scholar
- Adelman K, Marr MT, Werner J, Saunders A, Ni Z, Andrulis ED, Lis JT: Efficient release from promoter-proximal stall sites requires transcript cleavage factor TFIIS. Mol Cell. 2005, 17: 103-112. 10.1016/j.molcel.2004.11.028.PubMedView ArticleGoogle Scholar
- Muse GW, Gilchrist DA, Nechaev S, Shah R, Parker JS, Grissom SF, Zeitlinger J, Adelman K: RNA polymerase is poised for activation across the genome. Nat Genet. 2007, 39: 1507-1511. 10.1038/ng.2007.21.PubMedPubMed CentralView ArticleGoogle Scholar
- Zeitlinger J, Stark A, Kellis M, Hong JW, Nechaev S, Adelman K, Levine M, Young RA: RNA polymerase stalling at developmental control genes in the Drosophila melanogaster embryo. Nat Genet. 2007, 39: 1512-1516. 10.1038/ng.2007.26.PubMedPubMed CentralView ArticleGoogle Scholar
- Kim TH, Barrera LO, Zheng M, Qu C, Singer MA, Richmond TA, Wu Y, Green RD, Ren B: A high-resolution map of active promoters in the human genome. Nature. 2005, 436: 876-880. 10.1038/nature03877.PubMedPubMed CentralView ArticleGoogle Scholar
- Guenther MG, Levine SS, Boyer LA, Jaenisch R, Young RA: A chromatin landmark and transcription initiation at most promoters in human cells. Cell. 2007, 130: 77-88. 10.1016/j.cell.2007.05.042.PubMedPubMed CentralView ArticleGoogle Scholar
- Iyer VR, Horak CE, Scafe CS, Botstein D, Snyder M, Brown PO: Genomic binding sites of the yeast cell-cycle transcription factors SBF and MBF. Nature. 2001, 409: 533-538. 10.1038/35054095.PubMedView ArticleGoogle Scholar
- The Encode Project Consortium: Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project. Nature. 2007, 447: 799-816. 10.1038/nature05874.PubMed CentralView ArticleGoogle Scholar
- The ENCODE Project. [http://www.genome.gov/10005107]
- Lorincz MC, Schubeler D: RNA polymerase II: just stopping by. Cell. 2007, 130: 16-18. 10.1016/j.cell.2007.06.040.PubMedView ArticleGoogle Scholar
- Rasmussen EB, Lis JT: In vivo transcriptional pausing and cap formation on three Drosophila heat shock genes. Proc Natl Acad Sci USA. 1993, 90: 7923-7927. 10.1073/pnas.90.17.7923.PubMedPubMed CentralView ArticleGoogle Scholar
- Fujinaga K, Irwin D, Huang Y, Taube R, Kurosu T, Peterlin BM: Dynamics of human immunodeficiency virus transcription: P-TEFb phosphorylates RD and dissociates negative effectors from the trans-activation response element. Mol Cell Biol. 2004, 24: 787-795. 10.1128/MCB.24.2.787-795.2004.PubMedPubMed CentralView ArticleGoogle Scholar
- Giardina C, Perez-Riba M, Lis JT: Promoter melting and TFIID complexes on Drosophila genes in vivo. Genes Dev. 1992, 6: 2190-2200. 10.1101/gad.6.11.2190.PubMedView ArticleGoogle Scholar
- Schübeler D, MacAlpine DM, Scalzo D, Wirbelauer C, Kooperberg C, van Leeuwen F, Gottschling DE, O'Neill LP, Turner BM, Delrow J, Bell SP, Groudine M: The histone modification pattern of active genes revealed through genome-wide chromatin analysis of a higher eukaryote. Genes Dev. 2004, 18: 1263-1271. 10.1101/gad.1198204.PubMedPubMed CentralView ArticleGoogle Scholar
- Barski A, Cuddapah S, Cui K, Roh TY, Schones DE, Wang Z, Wei G, Chepelev I, Zhao K: High-resolution profiling of histone methylations in the human genome. Cell. 2007, 129: 823-837. 10.1016/j.cell.2007.05.009.PubMedView ArticleGoogle Scholar
- Weber M, Hellmann I, Stadler MB, Ramos L, Pääbo S, Rebhan M, Schubeler D: Distribution, silencing potential and evolutionary impact of promoter DNA methylation in the human genome. Nat Genet. 2007, 39: 457-466. 10.1038/ng1990.PubMedView ArticleGoogle Scholar
- Dillon SC, Zhang X, Trievel RC, Cheng X: The SET-domain protein superfamily: protein lysine methyltransferases. Genome Biol. 2005, 6: 227-10.1186/gb-2005-6-8-227.PubMedPubMed CentralView ArticleGoogle Scholar
- Kapranov P, Drenkow J, Cheng J, Long J, Helt G, Dike S, Gingeras TR: Examples of the complex architecture of the human transcriptome revealed by RACE and high-density tiling arrays. Genome Res. 2005, 15: 987-997. 10.1101/gr.3455305.PubMedPubMed CentralView ArticleGoogle Scholar
- Wu JQ, Du J, Rozowsky J, Zhang Z, Urban AE, Euskirchen G, Weissman S, Gerstein M, Snyder M: Systematic analysis of transcribed loci in ENCODE regions using RACE sequencing reveals extensive transcription in the human genome. Genome Biol. 2008, 9: R3-10.1186/gb-2008-9-1-r3.PubMedPubMed CentralView ArticleGoogle Scholar
- Bertone P, Stolc V, Royce TE, Rozowsky JS, Urban AE, Zhu X, Rinn JL, Tongprasit W, Samanta M, Weissman S, Gerstein M, Snyder M: Global identification of human transcribed sequences with genome tiling arrays. Science. 2004, 306: 2242-2246. 10.1126/science.1103388.PubMedView ArticleGoogle Scholar
- Kapranov P, Cheng J, Dike S, Nix DA, Duttagupta R, Willingham AT, Stadler PF, Hertel J, Hackermüller J, Hofacker IL, Bell I, Cheung E, Drenkow J, Dumais E, Patel S, Helt G, Ganesh M, Ghosh S, Piccolboni A, Sementchenko V, Tammana H, Gingeras TR: RNA maps reveal new RNA classes and a possible function for pervasive transcription. Science. 2007, 316: 1484-1488. 10.1126/science.1138341.PubMedView ArticleGoogle Scholar
- Margulies M, Egholm M, Altman WE, Attiya S, Bader JS, Bemben LA, Berka J, Braverman MS, Chen YJ, Chen Z, Dewell SB, Du L, Fierro JM, Gomes XV, Godwin BC, He W, Helgesen S, Ho CH, Irzyk GP, Jando SC, Alenquer ML, Jarvie TP, Jirage KB, Kim JB, Knight JR, Lanza JR, Leamon JH, Lefkowitz SM, Lei M, Li J, et al: Genome sequencing in microfabricated high-density picolitre reactors. Nature. 2005, 437: 376-380.PubMedPubMed CentralGoogle Scholar
- Brenner S, Johnson M, Bridgham J, Golda G, Lloyd DH, Johnson D, Luo S, McCurdy S, Foy M, Ewan M, Roth R, George D, Eletr S, Albrecht G, Vermaas E, Williams SR, Moon K, Burcham T, Pallas M, DuBridge RB, Kirchner J, Fearon K, Mao J, Corcoran K: Gene expression analysis by massively parallel signature sequencing (MPSS) on microbead arrays. Nat Biotechnol. 2000, 18: 630-634. 10.1038/76469.PubMedView ArticleGoogle Scholar
- Bainbridge MN, Warren RL, Hirst M, Romanuik T, Zeng T, Go A, Delaney A, Griffith M, Hickenbotham M, Magrini V, Mardis ER, Sadar MD, Siddiqui AS, Marra MA, Jones SJ: Analysis of the prostate cancer cell line LNCaP transcriptome using a sequencing-by-synthesis approach. BMC Genomics. 2006, 7: 246-10.1186/1471-2164-7-246.PubMedPubMed CentralView ArticleGoogle Scholar
- Leptin M: twist and snail as positive and negative regulators during Drosophila mesoderm development. Genes Dev. 1991, 5: 1568-1576. 10.1101/gad.5.9.1568.PubMedView ArticleGoogle Scholar