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Figure 3 | Genome Biology

Figure 3

From: Histone deacetylase inhibition accelerates the early events of stem cell differentiation: transcriptomic and epigenetic analysis

Figure 3

Expression patterns of selected genes in TSA-treated ES cells and EBs. mRNA levels of Nanog, Sall1, Nr0b1, Pdgfrb1, Mlf1 and Hoxa1 in: (a) ES cells treated with 50 nM TSA for 6 and 12 h. After 12 h of treatment, TSA was removed and cells were cultivated for an additional period of 6 and 12 h. (b) EBs 0, 4 and 8 days old. Control mRNA levels (0 h TSA/0 days EBs) were set to 1 and normalized with glyceraldehyde phosphate dehydrogenase. mRNA levels were analyzed with real-time PCR.

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