Figure 3
From: A pathway sensor for genome-wide screens of intracellular proteolytic cleavage
HTRA3 and 4 release GLUC activity from Actin-dNGLUC. (a) Actin-dNGLUC, DEVDG3 or DEVAG2F were co-transfected with GFP, caspase 9 or TBC1D10A and GLUC activity in SN was assayed after 30 h. TBC1D10A specifically releases dNGLUC from DEVDG3, but not DEVAG2F (RLU, relative light units). (b) HTRA3 and 4 release GLUC activity from Actin-dNGLUC. Caspase 8, caspase 9, HTRA3 and HTRA4 were co-transfected with Actin-dNGLUC and DEVDG2F and SN were analyzed for GLUC activity in SN after 30 h. HTRA3 and 4 release GLUC activity from Actin-dN and DEVDG2F, while caspases 8 and 9 released GLUC activity from DEVDG2F but not Actin.