Computational identification of obligatorily autocatalytic replicators embedded in metabolic networks

Table 1 List of investigated metabolic networks and their main properties

	Total number of metabolites	Number of producible metabolites (maximum scope)	Number of food molecules	Scope of input metabolites	Additional metabolites to include for maximum scope*	Reference
Escherichia coli	761	692	143	315	ATP	[25]
Heliobacter pylori	485	441	74	182	ATP	[45]
Staphylococcus aureus	644	543	83	194	ATP	[46]
Saccharomyces cerevisiae	672	667	101	342	ATP	[47]
Lactococcus lactis	508	477	92	190	ATP	[48]
Streptomyces coelicolor	601	562	104	267	ATP	[49]
Mycobacterium tuberculosis	830	642	87	235	ATP	[50]
Methanosarcina barkeri	628	566	70	161	ATP + NAD⁺	[51]
Geobacter sulfurreducens	541	406	41	82	ATP + NAD⁺ + THF + CoA	[52]
Synechocystis ^†	879	634	18	64	ATP + NAD⁺ + THF + CoA + sugar	^‡
Synechocystis ^§	879	662	29	99	ATP + NAD⁺ + THF + CoA	^‡
Minimal metabolism	68	68	11	11	ATP	[16]

*For a list of equivalent molecules that give the same scope see Additional data file 1. ^†Autotrophic growth. ^‡Heterotrophic growth. ^§See Additional data file 1 and Additional data file 3.

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