Table 3 Primer sequences
From: The impact of chromatin modifiers on the timing of locus replication in mouse embryonic stem cells
Replication timing/ChIP | |||
|---|---|---|---|
Locus | Forward | Reverse | Tann |
Gbe | GGTGCAGATCATCCCCTTGA | TTACCCGACGGCGAAAG | 60 |
α-Globin | CCACAAGCTGCGTGTGGAT | ATGCCGCCTGCCAGGT | 60 |
Oct4 | GGGTGAGAAGGCGAAGTCTGAA | GTGAGCCGTCTTTCCACCAGG | 55 |
Nanog | CCCTCTGAGTTTGACCGGTGA | CAAGCTAGGATGTTAGGTCTCCCTG | 60 |
Esg1 | AAAGACGAACACAGAGTCAAACACC | CACCTGCTCGATGTGAGACATTC | 60 |
Zfp57 | TGCAAGATAAGAACGAGGAGCAGGAG | CCTTTGCGGCTTTGTGGATTTGTG | 60 |
Rex1 | TTTGCGGGAATCCAGCAGT | CGTCCCATCGCCACTCTAGAC | 55 |
Sox2 | CCATCCACCCTTATGTATCCAAG | CGAAGGAAGTGGGTAAACAGCAC | 55 |
Nkx2.9 | AAGTGCGAGGCGCTCG | TGGCACCTTCCGGACTTG | 60 |
Sox3 | TGCCCAGATGGCTTCCTATT | ACCCGGACATTCTCCGCT | 60 |
Mage a2 | TTGGTGGACAGGGAAGCTAGGGGA | CGCTCCAGAACAAAATGGCGCAGA | 60 |
Ebf | AGATCTGGTTGAAGCCCTGTATGG | CATGTCACATCTCAGATCCTGTGTTCT | 60 |
Mash1 | CCAGGCTGGAGCAAGGGA | CGGTTGGCTTCGGGAGC | 55 |
β-Globin | GGTGAACTTTACTGCTGAGGAAAAG | TCACCACCAACCTCTTCAACAT | 60 |
Myf5 | GGAGATCCGTGCGTTAAGAATCC | CGGTAGCAAGACATTAAAGTTCCGTA | 55 |
Math1 | CCCTCACTCAGGTCGCCTG | CGTGCGAGGAGCCAATCA | 55 |
Sox1 | ACAAGAGGAGGCAGCGAACC | TCGCAGGTGGAAAGTTTCTCC | 55 |
Msx1 | ACAGAAAGAAATAGCACAGACCATAAGA | TTCTACCAAGTTCCAGAGGGACTTT | 55 |
Frg1 | AAGGAGCCTATATCCATGCACTGGAC | GCCTCCCTGCCATTGCTTGT | 60 |
Slc7a2 | GACAAGGAACAGGGCGAGAAG | CTTTCCTCATCCTGGGCTTGAGTA | 60 |
Msr1 | GCCACCAATGCCCTAGAATTTC | GGCAGGCTCTCACTAGGAAGC | 60 |
Loc2 | ACTAGCAACTGGACATAAGAGTACACTACC | ATTACATATGGTGTCTGGAAGCCAG | 60 |
Adam 26 | CCTTGAACAACGCCCTTTTGTG | GCAAGCTCCCAAAACAGGTGT | 60 |
Loc4 | TAAGGTAGGCAGTGAGAGACATCCA | GGTGTAAGAAGGTTAGAACTAA | 60 |
X141 | GGGTCATAAAACGCTTTTCCAGGAA | TAGCACTGGAGATCAGATTGACGCCT | 60 |
Minor satellite | TGATATACACTGTTCTACAAATCCCGTTTC | ATCAATGAGTTACAATGAGAAACATGGAAA | 55 |
Major satellite | GACGACTTGAAAAATGACGAAATC | CATATTCCAGGTCCTTCAGTGTGC | 55 |
rDNA | CCTGTGAATTCTCTGAACTC | CCTAAACTGCTGACAGGGTG | 60 |
IAP | TTGATAGTTGTGTTTTAAGTGGTAAATAAA | AAAACACCACAAACCAAAATCTTCTAC | 60 |
LINE 1 | TTTGGGACACAATGAAAGCA | CTGCCGTCTACTCCTCTTGG | 60 |
SINE B1 | GTGGCGCACGCCTTTAATC | GACAGGGTTTCTCTGTGTAG | 60 |
Gene expression | |||
Mage a2 | GAAGATCTCAGGAGTGTCAGGACTG | TCAGCCATTATGACTGTCCTAGGTAA | 60 |
Ubc | AGGAGGCTGATGAAGAGCTTGA | TGGTTTGAATGGATACTCTGCTGGA | 60 |
Oct4 | CCCAAGGTGATCCTCTTCTGCTT | GAGAAGGTGGAACCAACTCCCG | 60 |
CpG methylation assay | |||
Major sat | GACGACTTGAAAAATGACGAAATC | CATATTCCAGGTCCTTCAGTGTGC | 55 |
Sox2 | TGGACTGCGAACTGGAGAAGG | CGCCCGGAGTCTAGCTCTAAATATT | 60 |