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Table 1 BAC library fingerprinting summary

From: A physical map of the bovine genome

Library

Library indicator*

Library construction enzyme†

Source DNA

Clones fingerprinted

Successful fingerprints

Clones in FPC‡

Average size (kb)§

Clone depth

CHORI240

E

MboI

Hereford bull L1 Domino 99373

200,064

170,644

169,283

169

9.5X

RPCI42

H

EcoRI/EcoRI methylase

Holstein bull

94,848

83,627

81,437

171

4.6X

TAMBT

T

HindIII

Angus bull, Angus cow

44,928

40,380

40,077

106

1.4X

Total

   

339,840

294,651

290,797

161

15.5X

  1. *The one-letter prefix used in clone names to indicate the library source. †All libraries were constructed from partially digested DNA. ‡After filtering. §Based on the sum of the sizes of the HindIII fingerprint fragments identified for each clone, excluding vector-specific fragments.