Table 1 Primers used for determination of mRNA expression levels by Q-RT-PCR
Gene | Forward primer | Reverse primer |
|---|---|---|
Mouse | Â | Â |
Gapdh | TGCACCACCAACTGCTTAG | GATGCAGGGATGATGTTC |
Oct4 (Pou5f1) | CCAATCAGCTTGGGCTAGAG | CCTGGGAAAGGTGTCCTGTA |
Pecam | GTCATGGCCATGGTCGAGTA | CTCCTCGGCGATCTTGCTGAA |
Lyve-1 | AGGAGCCCTCTCCTTACTGC | ACCTGGAAGCCTGTCTCTGA |
vWF | GCCAAAGATCTGGAACAGTGT | GATGGAGAGGTTACACATCTC |
F2 | CAGCTATGAGGAGGCCTTTG | TCACACCCAGATCCATAGCA |
Tat | TTAAGTCCAATGCGGACCTC | GCTCTGTGAATTCCACGTCA |
Afp | GCCCTACAGACCATGAAACAAG | GTGAAACAGACTTCCTGGTCCT |
Ttr | CTTTGCCTCTGGGAAGACC | CAGAGTCGTTGGCTGTGAAA |
Foxa2 | CCCGGGACTTAACTGTAACG | TCATGTTGCTCACGGAAGAG |
Hnf4a | GGTCAAGCTACGAGGACAGC | ATGTACTTGGCCCACTCGAC |
Sox7 | CTTCAGGGGACAAGAGTTCG | CCATGACTTTCCCAGCATCT |
Sox17 | CACAACGCAGAGCTAAGCAA | TTGTAGTTGGGGTGGTCCTG |
Myocd | CTGTGTGGAGTCCTCAGGTCAAACC | GATGTGTTGCGGGCTCTTCAG |
Pdgra | ACGTTCAAGACCAGCGAGTT | CCTCCAGCATGGTGATACCT |
Pdgfrb | CACCTTCTCCAGTGTGCTGA | GGAGTCCATAGGGAGGAAGC |
Tek | AAGCATGCCCATCTGGTTAC | GCCTGCCTTCTTTCTCACAC |
Fbx15 | GGCCTTGAATGGAGAACTGA | TCAAACCACCCTAGGTCTGC |
Dnmt3l | CCTGGTGAAGAACTGCCTTC | GCAAAGTGAGCTGCACAGAG |
Rex1 | CCTGCACACAGAAGAAAGCA | TCAGTCTGTCGAGGGCTCTT |
Dppa5 | CAGTCGCTGGTGCTGAAATA | TCCATTTAGCCCGAATCTTG |
Eras | CCCTGCTTGTCCATGAGATT | TGGTAACTTGGTCGGAGAGG |
Utf1 | GGCCATACCTTCGAATCCTC | GGTTTGGTCGAAGGAACCTC |
Tdgf1 | GCATCCTACGAGGGAGTTGA | CACTGTGCTTGGCTGAAGAA |
Tcl1 | TTCCTCTCTGGGTGTTCAGG | ATCCCACACATTCCCTTTCA |
Ecat1 | GAATGCCTGGAAGATCCAAA | AAATCTCAGCTCGCCTTTCA |
Nanog | GAGTGTGGGTCTTCCTGGTC | GAGGCAGGTCTTCAGAGGAA |
Gdf3 | CGCAGGACTTATGCTACGTG | CTGGGCCATGGTCAACTT |
Fgf4 | CCGACGAGTGTAAATTCAAAGAA | GGAAGTGGGTTACCTTCATGG |
Sox2 | ACCAGCTCGCAGACCTACAT | AGTGGGAGGAAGAGGTAACCA |
Sox1 | CACAACTCGGAGATCAGCAA | TGTAATCCGGGTGTTCCTTC |
Pax6 | TCAGACCTCCTCATACTCGTGCA | TGTAGGTATCATAACTCCGCCCA |
Rat | Â | Â |
Gapdh | TGCACCACCAACTGCTTAG | GATGCAGGGATGATGTTC |
Oct4 (Pou5f1) | CTGTAACCGGCGCCAGAA | TGCATGGGAGAGCCCAGA |
Vecad | GGCCAACGAATTGGATTCTA | GTTTACTGGCACCACGTCCT |
Flt-1 | TGGCCAGAGGCATGGAGT | TCGCAAATCTTCACCACATTG |
Flk-1 | CCAAGCTCAGCACACAAAAA | CCAACCACTCTGGGAACTGT |
vWF | CCCACCGGATGGCTAGGTATT | GAGGCGGATCTGTTTGAGGTT |
Afp | ACCTGACAGGGAAGATGGTG | GCAGTGGTTGATACCGGAGT |
Alb | CTGGGAGTGTGCAGATATCAGAGT | GAGAAGGTCACCAAGTGCTGTAGT |
Tat | AACCTCAGCACCAATGTTCC | TCTTCAGAGCACCCTGGACT |
Ttr | CAGCAGTGGTGCTGTAGGAGTA | GGGTAGAACTGGACACCAAATC |
Sox2 | AACCCCAAGATGCACAACTC | CCGGGAAGCGTGTACTTATC |
Otx1 | GTTCGCAAAGACTCGCTACC | CCGGAGACGACTTCTTCTTG |
Blbp | CCAGCTGGGAGAAGAGTTTG | TTTCTTTGCCATCCCACTTC |
Pax6 | GTCCATCTTTGCTTGGGAAA | TAGCCAGGTTGCGAAGAACT |