From: Accuracy and quality of massively parallel DNA pyrosequencing
Data selection | Percent of reads | Error rate |
---|---|---|
All reads | 100.0% | 0.49% |
Reads with no Ns | 94.4% | 0.24% |
Reads with one or more Ns | 5.6% | 4.7% |
Reads with length ≥81 and ≤108 | 98.8% | 0.33% |
Reads with length <81 or >108 | 1.2% | 18.9% |
Reads with no Ns and length ≥81 and ≤108 | 93.3% | 0.20% |
Reads with no proximal errors | 97.0% | 0.45% |
Reads with fewer than three proximal errors | >99.99% | 0.48% |
Reads with more than three proximal errors | <0.01% | 12.2% |
Reads with no Ns and length ≥81 and ≤108 and no proximal errors | 90.6% | 0.16% |