Cloning and characterization of microRNAs from wheat (Triticum aestivum L.)

Yao, Yingyin; Guo, Ganggang; Ni, Zhongfu; Sunkar, Ramanjulu; Du, Jinkun; Zhu, Jian-Kang; Sun, Qixin

doi:10.1186/gb-2007-8-6-r96

Table 2 Conserved wheat miRNA families homologous to known miRNAs from other plant species

From: Cloning and characterization of microRNAs from wheat (Triticum aestivum L.)

miRNA family	Name	Sequence(5'-3')*	Length (nt)	Pri-miRNA EST no.	Conserved in other plants^†
					Rice	Arabidopsis	Maize	Sorghum
156/157	TaMIR156a	UGACAGAAGAGAGUGAGCAC	20	Not found	++	++	++	++
	TaMIR156k	UUGACAGAAGAGAGUGAGCA	20		+	+	+	+
	Ta MIR156m	UGACAGAAGAGAGUGAGCCU	20		+	+	+	+
159	TaMIR159a	UUUGGAUUGAAGGGAGCUCUG	21	CA731881	++	+	++	++
	TaMIR159b	UUUGGAUUGAAGGGAGCUCUU	21	CA484819 CA682604	+	++	+	+
160	TaMIR160	UGCCUGGCUCCCUGUAUGCCA	21	CJ641547	++	++	++	++
164	TaMIR164a	UGGAGAAGCAGGGUACGUGCA	21	CA704421	++	++	++	++
165	TaMIR165	UCGGACCAGGCUUCAUCCCC	20	Not found		+
166	TaMIR166b	UCGGACCAGGCUUCAUUCCC	20	Not found	++	++	++	++
	TaMIR166g	UCGGACCAGGCUUCAAUCCC	20		++	++	++	++
167	TaMIR167a	UGAAGCUGCCAGCAUGAUCUA	21	CK209908	++	++	++	++
	TaMIR167g	UGAAGCUGCCAGCAUGAUCUG	21	CK209889	++	++	++	++
	TaMIR167m	UGAAGCUGCCAGCAUGAUCUGA	22		+	+	+	+
168	TaMIR168a	UCGCUUGGUGCAGAUCGGGAC	21	Not found	++	+	++	++
	TaMIR168b	UCGCUUGGUGCAGAUCGGGAU	21		+	+	+	+
169	TaMIR169a	CAGCCAAGGAUGACUUGCCGA	21	BJ225371	++	++	++	++
	TaMIR169b	CAGCCAAGGAUGACUUGCCGG	21		++	++	++	++
	TaMIR169n	ACAGCCAAGGAUGACUUGCCG	21		+	+	+	+
	TaMIR169m	UAGCCAAGGAUGACUUGCCUG	21		++	++	++	++
	TaMIR169o	UAGCCAAGGAUGACUUGCCUA	21		++	++	++	++
170/171	TaMIR171a	UGAUUGAGCCGUGCCAAUAUC	21	CD910903	++	++	++	++
	TaMIR171b	UUGAGCCGUGCCAAUAUCACG	21		+	++	+	+
	TaMIR171h	GUGAGCCGAACCAAUAUCACU	21		++	+	++	++
172	TaMIR172a	AGAAUCUUGAUGAUGCUGCAU	21	Not found	++	++	++	++
	TaMIR172n	GAAUCUUGAUGAUGCUGCAU	20		+	+	+	+
	TaMIR172c	UGAAUCUUGAUGAUGCUGCAU	21		+	+	+	+
319	TaMIR319a	UUGGACUGAAGGGUGCUCCC	20	Not found	++	+	++	++
	TaMIR319d	UUUGGAUUGAAGGGAGCUCU	20	Not found
390	TaMIR390	AAGCUCAGGAGGGAUAGCGCC	21	Not found	++	++
393	TaMIR393	UCCAAAGGGAUCGCAUUGAUC	21	Not found	++	++	++	++
396	TaMIR396a	UUCCACAGCUUUCUUGAACUG	21	Not found	++	++	++	++
397	TaMIR397	UUGAGUGCAGCGUUGAUGAA	20	Not found	+	+
399	TaMIR399	UGCCAAAGGAGAAUUGCCC	19	CJ666653	+	+	+	+
408	TaMIR408	CUGCACUGCCUCUUCCCUGGC	22	BE419354	++	++	++
444	TaMIR444	UUGCUGCCUCAAGCUUGCUGC	21	CK200584	++
				CA596074
				BE405735
479	TaMIR479	AGUGAUAUUGGUCCGGCUCAUU	22	Not found

The sequences given in this table represent the longest miRNA sequences identified by cloning and 454 sequencing. *The underlined nucleotides represent the non-conserved nucleotides among wheat and other plant species. ^†The plus symbols indicate: ++, miRNA sequences of wheat were exactly identical to those in other species; +, miRNA sequences of wheat were conserved in other species but have variations in some nucleotide positions.

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