Table 1 Novel wheat miRNAs identified by direct cloning
From: Cloning and characterization of microRNAs from wheat (Triticum aestivum L.)
Name | Sequence | Length (nt) | EST no.* | Unigene | EST length | Precursor length | Start, end | Energy kcal mol-1 | Expression |
|---|---|---|---|---|---|---|---|---|---|
TamiR501 | UAGUACCGGUUCGUGGCACGAACC | 24 | CA718024 | Ta.23206 | 168 | 83 | 20, 102 | -67.20 | Not detected |
| Â | Â | Â | CD878657 | Ta.34663 | 551 | 151 | 92, 242 | -82.40 | Â |
TamiR502 | CACUACAUUAUGGAAUGGAGGGA | 23 | CA670378 | Ta.2228 | 550 | 245 | 216, 460 | -94.10 | Northern blot |
TamiR503 | UGGCACGGCGUGAUGCUGAGUCAG | 24 | BG262612 | Ta.14534 | 474 | 70 | 340, 409 | -36.3 | Not tested |
TamiR504 | ACAUUCUUAUAUUAUGAGACGGAG | 24 | CA739366 | Ta.28672 | 427 | 87 | 14, 100 | -68.6 | RT-PCR |
TamiR505 | AGUAGUGAUCUAAACGCUCUUA | 22 | BJ323011 | Ta.38265 | 690 | 87 | 248, 334 | -63.8 | RT-PCR |
| Â | Â | Â | BJ263967 | Ta.2752 | 464 | 115 | 78, 192 | -49.9 | Â |
| Â | Â | Â | CA694693 | Ta.12686 | 491 | 88 | 92, 180 | -41.4 | Â |
TamiR506 | UAGAUACAUCCGUAUCUAGA | 20 | CK214157 | Ta.32635 | 1,048 | 126 | 140, 265 | -89.3 | RT-PCR |
| Â | Â | Â | BE430261 | Ta.38727 | 558 | 128 | 292, 420 | -69.3 | Â |
| Â | Â | Â | BJ267812 | Ta.14358 | 179 | 129 | 10, 138 | -80.4 | Â |
TamiR507 | UCCGUGAGACCUGGUCUCAUAGA | 23 | CK217185 | Ta.30511 | 1,047 | 181 | 550, 730 | -82.4 | Northern blot |
| Â | Â | Â | AY747601 | - | - | 218 | 1, 218 | -154.3 | Â |
TamiR508 | GCAGGACGUGAAGAGCGAGUCC | 22 | BE417418 | Ta.23807 | 310 | 115 | 155, 269 | -52.70 | RT-PCR |
TamiR509 | AACCAACGAGACCAACUGCGGCGG | 24 | CA635339 | Ta.2228 | 583 | 179 | 190, 368 | -87.8 | Northern blot |
TamiR510 | UCCACUAUGGACUACAUACGGAG | 23 | AJ603161 | Ta.639 | 429 | 163 | 95, 257 | -70.1 | Not detected |
TamiR511 | UCCUUCCGUUCGGAAUUAC | 19 | BE405744 | Ta.30840 | 545 | 116 | 260, 375 | -42.3 | Not tested |
TamiR512 | UACUACUCCCUCCGUCCGAAA | 21 | BJ320481 | Ta.7082 | 439 | 133 | 90, 222 | -86.9 | Northern blot |
TamiR513 | CAGCGAGCCAGCGGAGACCGGCAG | 24 | BJ260462 | Ta.6068 | 572 | 298 | 220, 517 | -138.0 | Northern blot |
TamiR514 | CCUCCGUCUCGUAAUGUAAGACG | 23 | CA676805 | Ta.14883 | 625 | 113 | 20, 132 | -51.2 | Northern blot |
TamiR515 | UAGUACCGGUUCGUGGCUAACC | 22 | CA686406 | Ta.22812 | 544 | 67 | 333, 399 | -43.9 | Northern blot |
TamiR516 | AUAGCAAGGAUUGACAGACUG | 21 | BJ215780 | Ta.25530 | 608 | 551 | 50, 600 | -172.9 | Not tested |
TamiR517 | CAUAUACUCCCUCCGUCCGAAA | 22 | BJ276129 | Ta.33730 | 281 | 145 | 50, 194 | -76.9 | Not tested |
TamiR518 | CAACAACAACAAGAAGAAGAAGAU | 24 | BE442798 | Ta.8114 | 588 | 379 | 91, 469 | -145.1 | Not tested |
TamiR519 | CUGCGACAAGUAAUUCCGAACGGA | 24 | CA698039 | Ta.28713 | 429 | 109 | 72, 180 | -60.3 | Not tested |
| Â | Â | Â | DR092358 | Ta.41690 | 250 | 109 | 100, 208 | -64.0 | Â |
TamiR520 | UUGUCGCAGGUAUGGAUGUAUCUA | 24 | BE591362 | Ta.2140 | 463 | 106 | 145, 250 | -68.8 | Not tested |
TamiR521 | UAGUACAAAGUUGAGUCAUC | 20 | BJ237878 | Ta.3199 | 685 | 123 | 109, 231 | -70.0 | Not tested |
| Â | Â | Â | BQ172311 | Ta.12786 | 474 | 89 | 62, 150 | -60.9 | Â |
TamiR522 | GCUUAGAUGUGACAUCCUUAAAA | 23 | DR733919 | Ta.12590 | 930 | 147 | 300, 446 | -32.0 | Not tested |
TamiR523 | AGAGUAACAUACACUAGUAACA | 22 | BQ903908 | Ta.27907 | 636 | 207 | 423, 629 | -67.4 | Not tested |
TamiR524 | CAUUAUGGAACGGAAGGAG | 19 | BJ241591 | Ta.9978 | 328 | 90 | 141, 230 | -46.5 | Not tested |