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Table 1 Primers used in this study

From: Monitoring of gene knockouts: genome-wide profiling of conditionally essential genes

Primer name

Sequence

For defined library

   Up-BIO

5'-biotin-GAACTTCGAACTGCAGGTCGAC-3'

   Dn-BIO

5'-biotin-GTATAGGAACTTCGAAGCAGCTC-3'

   Up-Cy3

5'-GGTCGACGGACCCCCG-3'

   Up-Cy5

5'-GGTCAACGGATCCCCG-3'

   Dn-Cy3

5'-AAGCAGTTCCAGCCTACA-3'

   Dn-Cy5

5'-AAGCAGCTCCAGTCTACA-3'

For random library

   Tn10OE-BIO

5'-biotin-CAAGATGTGTATCCACCTTAACTTAA-3'

   Tn10OE OUT-Cy3

5'-ACCAATATCATTAGGGGAT-3'

   Tn10OE OUT-Cy5

5'-ACCAAAATCATAAGGGGAT-3'

Common for both libraries

   TATV-3 or Oligo(dT9 AT15V)

5'-T9 AT15V-3'

   TATV-5 or Oligo(dT15 AT9V)

5'-T15 AT9V-3'

  1. Mismatched nucleotides are in bold. 'V' represents A, C, or G.