From: Monitoring of gene knockouts: genome-wide profiling of conditionally essential genes
Primer name | Sequence |
---|---|
For defined library | |
   Up-BIO | 5'-biotin-GAACTTCGAACTGCAGGTCGAC-3' |
   Dn-BIO | 5'-biotin-GTATAGGAACTTCGAAGCAGCTC-3' |
   Up-Cy3 | 5'-GGTCGACGGACCCCCG-3' |
   Up-Cy5 | 5'-GGTCAACGGATCCCCG-3' |
   Dn-Cy3 | 5'-AAGCAGTTCCAGCCTACA-3' |
   Dn-Cy5 | 5'-AAGCAGCTCCAGTCTACA-3' |
For random library | |
   Tn10OE-BIO | 5'-biotin-CAAGATGTGTATCCACCTTAACTTAA-3' |
   Tn10OE OUT-Cy3 | 5'-ACCAATATCATTAGGGGAT-3' |
   Tn10OE OUT-Cy5 | 5'-ACCAAAATCATAAGGGGAT-3' |
Common for both libraries | |
   TATV-3 or Oligo(dT9 AT15V) | 5'-T9 AT15V-3' |
   TATV-5 or Oligo(dT15 AT9V) | 5'-T15 AT9V-3' |