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Figure 6 | Genome Biology

Figure 6

From: Genome-wide identification of novel expression signatures reveal distinct patterns and prevalence of binding motifs for p53, nuclear factor-κB and other signal transcription factors in head and neck squamous cell carcinoma

Figure 6

Basal and inducible gene expression, and promoter binding activity were modulated by doxorubicin or TNF-α. (a) Human keratinocyte (HKC) cells were treated with doxorubicin (Dox; 0.5 μg/ml, left panels), and the UM-SCC 6 cell line was treated with tumor necrosis factor (TNF)-α (2000 U/ml, center and right panels) for different periods, as indicated. Total RNA was harvested by Trizol and genes selected from clusters A to C were analyzed by real-time reverse transcription polymerase chain reaction (RT-PCR). The data are presented as the mean plus standard deviation from triplicates with normalization by 18S ribosome RNA. '(C1)', '(C2)', and '(C3)' refer to the three subclusters of cluster C. '(C)' refers to genes in cluster C outside subclusters C1 to C3. (b) Chromatin immunoprecipitation assays were performed in UM-SCC 11A cells using rabbit polyclonal anti-p65 or cRel antibodies with IgG isotype control.

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Genome Biology

ISSN: 1474-760X

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