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Table 2 Identifications of differentially expressed spots in mDCs, moDCs and KG-1 cells using MALDI-TOF mass spectrometry

From: Analysis of proteomic profiles and functional properties of human peripheral blood myeloid dendritic cells, monocyte-derived dendritic cells and the dendritic cell-like KG-1 cells reveals distinct characteristics

Spot no.

Protein identification

Accession no. (Swiss-Prot)

Induction factor* (mDC/moDC)

Induction factor* (mDC/KG-1)

Theoretical pI/Mr

Sequence coverage (%)

MASCOT score

Biological process†

1

Fibrinogen γ chain

P02679

8.60

3.28

5.37/51,511.66

17

91

1,8,12,13

2

Ubiquinol-cytochrome-c-reductase complex core protein 1

P31930

24.41

21.01

5.94/52,618.79

13

58

1,2

3

-

-

8.05

-2.27

-

   

4

-

-

Not in mo

21.06

-

   

5

Glutathione S-transferase P

P09211

2.49

1.42

5.44/23,224.64

40

102

1, 2, 9

6

L-lactate dehydrogenase B chain

P07195

1.70

-1.28

5.72/36,507.30

33

175

2

7

-

-

Not in m

Not in m

-

   

8

-

-

-4.06

9.51

-

   

9

Pyridoxal kinase

O00764

Not in m

Not in m

5.75/35,102.30

22

96

2

10

Nuclease isoform Sm3

P13717

1.54

2.01

6.95/28,944.65

56

191

1, 2

11

Annexin A1

P04083

1.43

2.26

6.64/38,583.05

59

202

1, 4, 5, 7, 9

12

Fructose-1,6-bisphosphatase

P09467

-14.34

2.62

6.54/36,818.12

48

205

1, 2

13

Fascin

Q16658

-8.78

-7.87

6.81/54,398.81

23

133

4,5,6

14

Phosphoglycerate mutase I

P18669

-1.37

-2.21

6.75/28,672.74

47

143

1, 2

15

Triosephosphate isomerase

P60174

1.02

-1.14

6.51/26,538.30

56

209

2

16

Actin related protein 2/3 complex subunit

O15144

-16.74

-20.03

6.84/34,333.02

19

91

4,5,6, 9

17

Carbonic anhydrase II

P00918

-15.63

-10.99

6.86/29,114.86

29

88

1, 2

18

Phosphoglucomutase I

Q16106

-33.05

-2.51

5.33/11,328.66

28

40

2

19

Cystatin B

P04080

-30.68

-2.13

6.96/11,139.59

34

61

1, 2, 9

20

-

-

-7.27

-3.69

-

   

21

-

-

-3.03

-22.70

-

   

22

Chain A, crystal structure of human Dj-1

Q99497

1.85

-2.02

6.33/19,891.05

36

89

1, 7

23

Proteasome beta 2 subunit

Q9BWZ9

-1.07

-5.82

6.52/22,840.06

26

77

1,2,9

24

dUTP pyrophosphatase isoform 2

P33316

Not in m or mo

Not in m or mo

9.65/26,706.39

35

93

1,2, 9

  1. MASCOT scores >64 were taken to be significant (p < 0.05). *Induction factor corresponds to the factor of difference between spot volume in mDCs compared with the respective mDC model. †Biological process: 1, cell growth/maintenance; 2, metabolism; 3, cell communication; 4, morphogenesis; 5, response to external stimulus; 6, cell motility; 7, response to stress; 8, circulation; 9, regulation of cellular processes; 10, cell differentiation; 11, death; 12, cell death; 13, coagulation; 14, homeostasis.