qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data

Table 2 Effects of the number and selection of IRCs on the increase in error and the fold difference between calibrated NRQs

	Increase in error		Fold difference between calibrated normalized quantities
	Mean [95% CI]	Max	Mean [95% CI]	Max
1 IRC
run1-run2	1.684 [1.579,1.797]	10.98	1.048 [1.034,1.061]	1.143
run1-run3	1.68 [1.576,1.79]	10.98	1.053 [1.038,1.067]	1.135
2 IRCs
run1-run2	1.374 [1.289,1.466]	7.73	1.024 [1.017,1.03]	1.069
run1-run3	1.489 [1.415,1.567]	7.73	1.026 [1.019,1.033]	1.065
3 IRCs
run1-run2	1.399 [1.292,1.513]	5.28
run1-run3	1.394 [1.288,1.508]	5.28

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