Figure 1

Comparison of histone H3 and H4 modifications in different cell types. (a) Purification of crude nucleosomal histones. Nucleosomal histones were separated on a 4-12% gradient NuPAGE gel and run in MES buffer (Invitrogen), fixed, and stained with Seeblue (Invitrogen). Lane 1, SeeBlue pre-stained molecular weight markers (Invitrogen); lane 2, nucleosomal histones from normal mouse liver; lane 3, nucleosomal histones from HeLa cells, purified on a POROS HQ column. (b) Methylation states of H3K9, H3K27, H3K36, and H4K20. nm, non-modified; me, monomethyl; me2, dimethyl; me3, trimethyl. Shown are the means of four independent experiments. (c) Basal amino-terminal modifications of histone 4 in the indicated cell types. 'H4 4-17nm': unmodified H4 peptide containing amino acids 4-17. Shown are the means of four independent experiments (± standard deviation). Pan-ac: panacetylated.