Figure 5

Anti-SREBP-2 antibody specifically enriches human PS2 DNA sequences in HepG2 cells in a ChIP assay. DNA precipitates were measured by real-time polymerase chain reaction using primers spanning the indicated regions. Control region (control) corresponds to the first coding exon of the neighboring gene SPBC24, which is approximately 7.5 kilobases away from the PS2 sequence. The results are presented as fold increase in the enrichment of precipitated DNA by anti-SREBP-2 (anti-sterol regulatory element binding protein-2) antibody over nonspecific IgG. Graphical representations of the mean ± standard error from three independent experiments are shown.